RNA
ASTERISK
Part:BBa_K4877000:Design
Designed by: Saint Fischer Group: iGEM23_Bielefeld-CeBiTec (2023-10-05)
ASTERISK: ADAR based precise detection and expression tool
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 826
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 775
Illegal BamHI site found at 1085
Illegal XhoI site found at 818 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2114
Design Notes
To make sure the ASTERISK-RNA works as it is supposed to be, we made sure the Sensor-RNA itself is free from restriction cloning scars. To provide a working and approachable System for other teams we designed this basic part as working out of the box. For this we describe in vitro translation and transfection based on lipid nano particels.
Source
ASTERISK works by using the MCP-ADAR2DD(E488Q)-NES enzyme found in human cells. This enzyme binds to a MS2 RNA hairpin derived from Bacteriophage MS2. The example RNA described in this Part contains sequences for mCherry, mNeonGreen.