Coding

Part:BBa_K4865001

Designed by: Jing Shi   Group: iGEM23_OTIA-Hangzhou   (2023-08-24)


R Codon Optimization (repeat region sequence of PySp1)

R is the DNA sequence of a repetitive region of Pyriform Spidroin1 (PySp1) from the piriform glandular filament of Arachnid. The function of the repeat area is mainly related to the mechanical properties of spider silk fiber. The sequence was optimized for prokaryotic expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None