Coding

Part:BBa_K4863002:Design

Designed by: Zhihan Emma Chen   Group: iGEM23_Keystone   (2023-10-09)


S-layer protein (Slp)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3112
    Illegal EcoRI site found at 5092
    Illegal XbaI site found at 318
    Illegal PstI site found at 434
    Illegal PstI site found at 4034
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3112
    Illegal EcoRI site found at 5092
    Illegal NheI site found at 741
    Illegal NheI site found at 838
    Illegal PstI site found at 434
    Illegal PstI site found at 4034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3112
    Illegal EcoRI site found at 5092
    Illegal BglII site found at 2253
    Illegal BamHI site found at 3159
    Illegal BamHI site found at 4473
    Illegal XhoI site found at 376
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3112
    Illegal EcoRI site found at 5092
    Illegal XbaI site found at 318
    Illegal PstI site found at 434
    Illegal PstI site found at 4034
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3112
    Illegal EcoRI site found at 5092
    Illegal XbaI site found at 318
    Illegal PstI site found at 434
    Illegal PstI site found at 4034
    Illegal AgeI site found at 2140
    Illegal AgeI site found at 2323
    Illegal AgeI site found at 2671
    Illegal AgeI site found at 2731
    Illegal AgeI site found at 4252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Surface display can be achieved via fusion to N-terminus of Slp.


Source

Synechocystis PCC6803

References