Coding

Part:BBa_K4854007

Designed by: iGEM23_NYCU-Formosa   Group: iGEM23_NYCU-Formosa   (2023-09-03)

Aacp19k

Barnacle cement protein Aacp19k is an adhesive protein from the striped barnacle (Amphibalanus amphitrite). At the cyprid stage before a barnacle becomes mature, the cyprid releases cement protein to anchor itself to substrates like rocks. [1]
We performed codon optimization to improve gene expression in E. coli. This basic part is used in BBa_K4854021 as a fusion protein with CsgA protein (BBa_K4854013).


Reference
[1] Liang Chao, Strickland Jack, Ye Zonghuang, Wu Wenjian, Hu Biru, Rittschof Dan, Biochemistry of Barnacle Adhesion: An Updated Review, Frontiers in Marine Science, Volume 6, 2019, URL: https://www.frontiersin.org/articles/10.3389/fmars.2019.00565


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
    Illegal AgeI site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None