Coding

Part:BBa_K4854006

Designed by: iGEM23_NYCU-Formosa   Group: iGEM23_NYCU-Formosa   (2023-09-03)

Mrcp20k

Barnacle cement protein Mrcp20k is an adhesive protein from the barnacle (Megabalanus rosa). At the cyprid stage before a barnacle becomes mature, the cyprid releases cement protein to anchor itself to substrates like rocks.[1]
We performed codon optimization to improve gene expression in E. coli. This basic part is used in BBa_K4854020 as a fusion protein with CsgA protein (BBa_K4854013).


Reference
[1] He, Li-Sheng et al. “Characterization of two 20kDa-cement protein (cp20k) homologues in Amphibalanus amphitrite." PloS one vol. 8,5 e64130. 22 May. 2013, doi:10.1371/journal.pone.0064130.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None