Coding
cp19k

Part:BBa_K4854005

Designed by: iGEM23_NYCU-Formosa   Group: iGEM23_NYCU-Formosa   (2023-09-03)

cp19k

Barnacle cement protein cp19k is an adhesive protein from the barnacle (Balanus albicostatus). At the cyprid stage before a barnacle becomes mature, the cyprid releases cement protein to anchor itself to substrates like rocks.[1]
We performed codon optimization to improve gene expression in E. coli. This basic part is used in BBa_K4854019 as a fusion protein with CsgA protein (BBa_K4854013).


Reference
[1] Fei Li, Luona Ye, Longyu Zhang, Xiaoyan Li, Xiaoxiao Liu, Jiarui Zhu, Huanhuan Li, Huimin Pang, Yunjun Yan, Li Xu, Min Yang, Jinyong Yan, Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive, Materials Today Bio, Volume 14, 2022.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
    Illegal AgeI site found at 295
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None