Coding

Part:BBa_K4854000

Designed by: iGEM23_NYCU-Formosa   Group: iGEM23_NYCU-Formosa   (2023-09-03)

Mfp3

Mussel foot protein Mfp3 is an adhesive protein from the Mediterranean mussel (Mytilus galloprovincialis). Mussels can secrete special proteins to adhere to surfaces under turbulent environments.[1]
We performed codon optimization to improve gene expression in E. coli. This basic part is used in BBa_K4854014 as a fusion protein with CsgA protein (BBa_K4854013).


Reference
[1] Zhong, C., Gurry, T., Cheng, A. et al. Strong underwater adhesives made by self-assembling multi-protein nanofibres. Nature Nanotech 9, 858–866 (2014).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 113
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None