Part:BBa_K4853006
nisC reverse primer
Primer designed for Lactococcus lactis end of nisC sequence.
Usage and Biology
Figure 1. Restriction digest to verify nisC primers. Beside a 1kb + ladder, the first lane contains a negative control, in which pACYCDuet-1 digested with Ssp1. The second lane contains miniprepped pACYCDuet-1-nisC digested with Ssp1 with a band at 1274 bp, verifying that nisC reverse primer was effective. Gel electrophoresis was conducted to separate the restriction digest products in a 1% agarose-TAE gel. The red asterisk highlights the nisC gene at 1274 bp.
A restriction digest was conducted to verify our plasmid, pACYCDuet-1, contained our gene of interest nisC. A DNA band was observed at the expected molecular weight, 1274 bp, indicating we were able to successfully clone DNA into our plasmid and that our nisC reverse primer is effective. This band is indicated by the red asterisk in Figure 1.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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