Part:BBa_K4853005
nisC forward primer
Primer designed for Lactococcus lactis beginning of nisC sequence.
Usage and Biology
Figure 1. Restriction digest to verify nisC primers. Beside a 1kb + ladder, the first lane contains a negative control, in which pACYCDuet-1 is digested with Ssp1. The second lane contains mini prepped pACYCDuet-1-nisC digested with Ssp1 with a band at 1274 bp, verifying that nisC forward primer was effective. Gel electrophoresis was conducted to separate the restriction digest products in a 1% agarose-TAE gel. The red asterisk highlights the nisC gene at 1274 bp.
A restriction digest was conducted to verify that our plasmid, pACYCDuet-1, contained our gene of interest nisC. A DNA band was observed at the expected band length, 1274 bp, indicating we were able to successfully clone DNA into our plasmid and that our nisC forward primer was effective. This band is indicated by the red asterisk in Figure 1.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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