Composite
Part:BBa_K4852048:Design
Designed by: Shen, Sean Chuan-En Group: iGEM23_GEMS-Taiwan (2023-10-12)
The metJ construct regulate production of biofilm.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2776
Illegal PstI site found at 2917 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 207
Illegal NheI site found at 230
Illegal NheI site found at 2561
Illegal NheI site found at 2584
Illegal NheI site found at 3105
Illegal NheI site found at 3128
Illegal PstI site found at 2776
Illegal PstI site found at 2917 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1863
Illegal XhoI site found at 2418
Illegal XhoI site found at 3948
Illegal XhoI site found at 4063
Illegal XhoI site found at 4618
Illegal XhoI site found at 4702
Illegal XhoI site found at 5257 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2776
Illegal PstI site found at 2917 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2776
Illegal PstI site found at 2917
Illegal NgoMIV site found at 775
Illegal NgoMIV site found at 2848
Illegal AgeI site found at 1331
Illegal AgeI site found at 2238
Illegal AgeI site found at 4438
Illegal AgeI site found at 5077 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1866
Illegal BsaI.rc site found at 3951
Illegal BsaI.rc site found at 4066
Illegal BsaI.rc site found at 4705
Design Notes
Gibson Assembly
Source
E. coli