Part:BBa_K4852048
The metJ construct regulate production of biofilm.
The metJ construct regulate production of biofilm.
The MetJ construct is responsible for regulating biofilm. The regulatory protein MetJ suppresses biofilm production, and by binding to the metbox operator, MetJ effectively reduces the synthesis of biofilm. We utilized the MetJ regulatory protein in a quorum sensing mechanism, a regulatory protein that represses the production of biofilm by attaching to the met operator. As the bacterial population increases over time, so does the concentration of the autoinducer (AHL). Autoinducers are signaling molecules that are produced in response to changes in cell population density. As the density of quorum-sensing bacterial cells increases so does the concentration of the autoinducer. Under conditions of low population density, there will be a low concentration of AHL, resulting in the low production of metJ proteins. Due to the low binding affinity of metJ, the production of biofilm under these conditions will be high. Under high population density, the high AHL concentration will induce the production of metJ, which will then decrease biofilm production, allowing the effective control of the gene expression responsible for biofilm formation. A CinR-CinI-CinS system further regulates MetJ synthesis and AHL production based on changes in population density. The CinR-CinI-CinS system is regulated through quorum sensing, which leads to the production of the MetJ regulatory protein. The main function of CinR is to control quorum sensing by initiating the expression of the cinIS operon. Meanwhile, the primary role of CinI is to synthesize AHL, while CinS works by inactivating PraR, a repressor molecule, through the formation of a 1:1 dead-end complex. The PraR repressor inhibits the activity of the praiI promoter, resulting in decreased production of MetJ as a consequence of reduced RaiI and RaiR levels. AHL synthesis is primarily carried out by RaiI, while RaiR functions as an AHL receptor that allows bacteria to respond to changes in population density. This response includes increased expression of CinI induced by AHL, resulting in higher levels of CinS expression, ultimately leading to increased MetJ expression. Furthermore, AHL can also be synthesized through the activity of the CinR-CinI-CinS system.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2776
Illegal PstI site found at 2917 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 207
Illegal NheI site found at 230
Illegal NheI site found at 2561
Illegal NheI site found at 2584
Illegal NheI site found at 3105
Illegal NheI site found at 3128
Illegal PstI site found at 2776
Illegal PstI site found at 2917 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1863
Illegal XhoI site found at 2418
Illegal XhoI site found at 3948
Illegal XhoI site found at 4063
Illegal XhoI site found at 4618
Illegal XhoI site found at 4702
Illegal XhoI site found at 5257 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2776
Illegal PstI site found at 2917 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2776
Illegal PstI site found at 2917
Illegal NgoMIV site found at 775
Illegal NgoMIV site found at 2848
Illegal AgeI site found at 1331
Illegal AgeI site found at 2238
Illegal AgeI site found at 4438
Illegal AgeI site found at 5077 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1866
Illegal BsaI.rc site found at 3951
Illegal BsaI.rc site found at 4066
Illegal BsaI.rc site found at 4705
| None |