Protein_Domain

Part:BBa_K4850006:Design

Designed by: Chai Chin Wei   Group: iGEM23_NUS-Singapore   (2023-10-10)


Rigid Protein Linker A(EAAAK)2A




Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The increase in the linker length was found to reduce the fluorescence resonance energy, as stated in Arai et al. (2001).


Source

The sequence of this rigid protein linker was obtained from the Arai et al. (2001) paper.


References

[1] Arai, R., Ueda, H., Kitayama, A., Kamiya, N., & Nagamune, T. (2001). Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Engineering Design & Selection, 14(8), 529–532. https://doi.org/10.1093/protein/14.8.529

[2] Chen, X., Zaro, J. L., & Shen, W. (2013). Fusion protein linkers: Property, design and functionality. Advanced Drug Delivery Reviews, 65(10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039