Coding

Part:BBa_K4849027:Design

Designed by: Devansh Kumar and Catarina Almeida   Group: iGEM23_Edinburgh   (2023-10-12)


MtrCAB - cytochrome protein complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 232


Design Notes

We extracted the Gene from Shewanella oneidensis MR-1 (NCBI:txid211586) genomic DNA using Primers that introduced cynogate assmbly system overhangs (refer IGEM Edinburgh 2023 wiki)

MtrC Forward Primer: CAGTgaagacATaATGATGAACGCACAAAAATC MtrB Reverse Primer: CAGTgaagacATaagcTTAGAGTTTGTAACTCATGCTC



Source

Shewanella oneidensis MR-1 (NCBI:txid211586)

References