Part:BBa_K4849004
NuiA antitoxin from Anabaena sp. PCC 7120
Description
NuiA is a specific inhibitor of the NucA nuclease from the cyanobacterium Anabaena sp. PCC 7120 (Ghosh et al., 2005). NucA and NuiA form a 1:1 complex leading to complete inhibition of NucA (Meiss et al., 1998) (Figure 1). The inhibition involves an unusual divalent metal ion bridge between the nuclease with its inhibitor (Ghosh et al., 2007). The C-terminal Thr-135 hydroxyl oxygen in NuiA interacts directly with the catalytic Mg2+ in the nuclease active site, while Glu-24 in NuiA extends into the active site, mimicking the charge of a scissile phosphate (Ghosh et al., 2007). NuiA residues Asp-75 and Trp-76 contribute to the strength and specificity of the interaction (Ghosh et al., 2007).
Figure 1. NucA-NuiA complex. Taken from Ghosh et al. (2007).
References
Ghosh, M., Meiss, G., Pingoud, A., London, R.E. and Pedersen, L.C., 2005. Structural insights into the mechanism of nuclease A, a ββα metal nuclease from Anabaena. Journal of Biological Chemistry, 280(30), pp.27990-27997.
Ghosh, M., Meiss, G., Pingoud, A.M., London, R.E. and Pedersen, L.C., 2007. The nuclease a-inhibitor complex is characterized by a novel metal ion bridge. Journal of Biological Chemistry, 282(8), pp.5682-5690.
Meiss, G., Franke, I., Gimadutdinow, O., Urbanke, C. and Pingoud, A., 1998. Biochemical characterization of Anabaena sp. strain PCC 7120 non‐specific nuclease NucA and its inhibitor NuiA. European journal of biochemistry, 251(3), pp.924-934.
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