Plasmid

Part:BBa_K4845011:Design

Designed by: Qian Xin   Group: iGEM23_SubCat-China   (2023-07-11)


XII-5-GA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4866
    Illegal XbaI site found at 5235
    Illegal SpeI site found at 3982
    Illegal PstI site found at 927
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3982
    Illegal PstI site found at 927
    Illegal NotI site found at 3761
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5658
    Illegal XhoI site found at 6451
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4866
    Illegal XbaI site found at 5235
    Illegal SpeI site found at 3982
    Illegal PstI site found at 927
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4866
    Illegal XbaI site found at 5235
    Illegal SpeI site found at 3982
    Illegal PstI site found at 927
    Illegal AgeI site found at 4326
    Illegal AgeI site found at 5697
    Illegal AgeI site found at 6578
    Illegal AgeI site found at 6603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2756
    Illegal BsaI site found at 6590
    Illegal BsaI.rc site found at 237
    Illegal SapI site found at 389
    Illegal SapI.rc site found at 1734


Design Notes

no


Source

no

References