Coding

Part:BBa_K4841010:Design

Designed by: Wu Dinghao   Group: iGEM23_TSBC-SZ   (2023-08-12)


SRRz


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

from bacteriophage l with a series of tightly controlled UV- and heat-inducible promoters to construct E.coli autolytic vectors.

References

1. Cai, Z., Xu, W., Xue, R., & Lin, Z. (2008). Facile, reagentless and in situ release of Escherichia coli intracellular enzymes by heat-inducible autolytic vector for high-throughput screening. Protein engineering, design & selection: PEDS, 21(11), 681–687.
2. Kloos, D. U., Strätz, M., Güttler, A., Steffan, R. J., & Timmis, K. N. (1994). Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death. Journal of bacteriology, 176(23), 7352–7361.