Coding

Part:BBa_K4836000:Design

Designed by: A Adithya Hitesh, M V P S Sriyanth, Sanchi Chaware, G Manonmani, Dhruvi Panchal, Akhshaya Mohan   Group: iGEM23_IISER-Tirupati-India   (2023-10-09)


CUW_0748: Bacterial analogue of Human Serotonin Transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the origin of the CDS is Turicibacter sanguinis, it was codon optimized for Escherichia coli and after optimization, BglII restriction site was removed to make it compatible for RCF21 assembly standard.


Source

The Coding sequence(CDS) of this part is obtained from NCBI Genbank, (Accession number - ADMN01000065).

References