Coding
Part:BBa_K4835034:Design
Designed by: Kun Zhang Group: iGEM23_XJU-China (2023-10-11)
The protein-coding gene thrC can be used to translate threonine synthase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 109
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 109
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 109
Illegal XhoI site found at 1277 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 109
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 109
Illegal NgoMIV site found at 1239
Illegal AgeI site found at 252
Illegal AgeI site found at 409 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 217
Illegal SapI.rc site found at 598
Illegal SapI.rc site found at 937
Design Notes
If you want to use it in a project, you need to be careful not to affect other genes.
Source
Reference Genome: Enterobacter cloacae RS35