Coding

Part:BBa_K4835033:Design

Designed by: Kun Zhang   Group: iGEM23_XJU-China   (2023-10-11)


The protein-coding gene thrC can be used to translate threonine synthase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
    Illegal PstI site found at 1175
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 346
    Illegal PstI site found at 1175
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
    Illegal PstI site found at 1175
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
    Illegal PstI site found at 1175
    Illegal AgeI site found at 430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 121
    Illegal SapI.rc site found at 1318


Design Notes

If you want to use it in a project, you need to be careful not to affect other genes.


Source

Reference Genome: Halomonas campaniensis LS21

References