Coding
Part:BBa_K4835032:Design
Designed by: Kun Zhang Group: iGEM23_XJU-China (2023-10-11)
The protein-coding gene thrC can be used to translate threonine synthase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 335
Illegal XhoI site found at 415
Illegal XhoI site found at 859 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 298
Illegal NgoMIV site found at 568
Illegal NgoMIV site found at 919
Illegal NgoMIV site found at 963
Illegal NgoMIV site found at 967 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
If you want to use it in a project, you need to be careful not to affect other genes.
Source
Reference Genome: Micrococcus luteus R17