Part:BBa_K4833024

This sequence expresses two enzymes crucial for DAP synthesis in the presence of Arabidose.

This sequence consists of four parts: the Arabidose operon, the murA gene, the SD sequence, and the asdA sequence. In the presence of Arabidose, the Arabidose operon can initiate the transcription of the murA gene, SD sequence, and asdA sequence. In the absence of Arabidose, the Arabidose operon cannot initiate transcription of downstream genes. asdA is a relevant gene that regulates the synthesis of diaminopimelic acid (DAP), which is an essential component of bacterial cell wall peptidoglycan. When the asdA gene is knocked out in attenuated Salmonella X11802, bacteria are growth-limited due to the inability to synthesize DAP. However, normal bacterial growth can be restored by adding extra DAP. murA is a relevant gene that regulates the synthesis of cell wall acid, which is also an essential component of bacterial cell wall peptidoglycan. When the murA gene is knocked out in attenuated Salmonella X11802, bacteria are growth-limited due to the inability to synthesize cell wall acid. However, by reinserting the murA gene controlled by the Arabidose promoter, normal bacterial growth can be achieved when Arabidose is added, allowing for the synthesis of cell wall acid. However, as Arabidose is depleted, the murA gene controlled by the Arabidose promoter ceases to be expressed, leading to bacterial lysis. Due to the cell death and lysis caused by the aforementioned mutations, additional DAP and Arabidose need to be supplemented when culturing attenuated Salmonella X11802 in vitro. In summary, this sequence is essential for bacterial synthesis of DAP when Arabidose is present. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1128
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1067
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 909
  Illegal AgeI site found at 1275
  Illegal AgeI site found at 1867
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2893
  Illegal SapI site found at 891