Composite

Part:BBa_K4833008:Design

Designed by: Xuanxu Piao   Group: iGEM23_JLU-NBBMS   (2023-10-11)


shSTAT3/shPD-L1 transcribes shSTAT3 and shPD-L1, silencing STAT3 and PD-L1 gene expression.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 400
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 84


Design Notes

1 Due to the polyT structure being a termination signal for RNA polymerase III, it is necessary to avoid having four or more consecutive T's in the shRNA sequence. 2 Avoid target sites that overlap with single nucleotide polymorphism (SNP) regions. 3 The U6 promoter can only transcribe relatively small gene sequences (mostly gene fragments less than 100bp), and cannot transcribe protein-coding genes



Source

Our Team designed

References