Coding

Part:BBa_K4830031:Design

Designed by: Beibitkyzy Aruzhan   Group: iGEM23_NTU-Singapore   (2023-10-11)


BLV RT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.

Source

NCBI ACCESSION: P03361

References

Sagata, N. et al. (1985) ‘Complete nucleotide sequence of the genome of bovine leukemia virus: Its evolutionary relationship to other retroviruses.’, Proceedings of the National Academy of Sciences, 82(3), pp. 677–681. doi:10.1073/pnas.82.3.677.