Coding

Part:BBa_K4830030:Design

Designed by: Beibitkyzy Aruzhan   Group: iGEM23_NTU-Singapore   (2023-10-11)


MiniRT2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.

Source

NCBI ACCESSION: AFC40210

References

Biđin, M. et al. (2014) ‘Genetic characterization of hepadnaviruses associated with histopathological changes in the liver of duck and goose embryos’, Veterinary Microbiology, 174(3–4), pp. 302–308. doi:10.1016/j.vetmic.2014.09.026.