Part:BBa_K4830027:Design
mCherry_C-terminus stop codon_Clover
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 352
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 352
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 352
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 352
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Given that the stop codon is installed at the C-terminus of the mCherry coding sequence, the mCherry protein is still expressed while the GFP is not expressed. This is particularly useful in assays that still require the presence of mCherry for detection.
Source
The mCherry fluorescent protein is derived from Discosoma sp. It has an excitation maxima at 587nm, and emission maxima of 610nm.
The Clover fluorescent protein (GFP) is derived from Aequorea victoria. It has an excitation maxima of 498nm, and emission maxima of 509nm.
References
Certo, M. T., Ryu, B. Y., Annis, J. E., Garibov, M., Jarjour, J., Rawlings, D. J., & Scharenberg, A. M. (2011). Tracking genome engineering outcome at individual DNA breakpoints. Nature Methods, 8(8), 671–676.