RNA
Part:BBa_K4829022:Design
Designed by: Mrigank Pawagi, Das Labs, Aditya Kamath Ammembal Group: iGEM23_IISc-Bengaluru (2023-10-09)
Ribotree optimised sequence for the CDS of BBa_K4829015
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 300
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 300
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 300
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 300
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 300
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These sequences were optimised by software. The one thing to keep in mind is that it can't be used to optimise UTRs, as they are, very often, not multiples of three, and Ribotree attempts codon optimisation. (However, in UTRs, there are no codons!)
Source
This part is not derived from any organism to the best of our knowledge