Plasmid
Part:BBa_K4828018:Design
Designed by: Yota Kashima Group: iGEM23_TokyoTech (2023-10-10)
pCMV-NCre
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 863
Illegal PstI site found at 1248
Illegal PstI site found at 3078
Illegal PstI site found at 4266 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 863
Illegal PstI site found at 1248
Illegal PstI site found at 3078
Illegal PstI site found at 4266 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 863
Illegal PstI site found at 1248
Illegal PstI site found at 3078
Illegal PstI site found at 4266 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 863
Illegal PstI site found at 1248
Illegal PstI site found at 3078
Illegal PstI site found at 4266
Illegal NgoMIV site found at 2643
Illegal NgoMIV site found at 3397
Illegal NgoMIV site found at 4717
Illegal NgoMIV site found at 5000 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2608
Illegal SapI.rc site found at 4566
Illegal SapI.rc site found at 4776
Design Notes
After meeting with the PI we decided to use the CMV promoter which is a strong promoter. We also expanded the screening selectivity by incorporating a kanamycin/neomycin resistance gene.
Source
the parts created by 2022 iGEM Tokyo Tech, BBa_K4419025.