Plasmid

Part:BBa_K4828018:Design

Designed by: Yota Kashima   Group: iGEM23_TokyoTech   (2023-10-10)


pCMV-NCre


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 863
    Illegal PstI site found at 1248
    Illegal PstI site found at 3078
    Illegal PstI site found at 4266
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 863
    Illegal PstI site found at 1248
    Illegal PstI site found at 3078
    Illegal PstI site found at 4266
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 863
    Illegal PstI site found at 1248
    Illegal PstI site found at 3078
    Illegal PstI site found at 4266
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 863
    Illegal PstI site found at 1248
    Illegal PstI site found at 3078
    Illegal PstI site found at 4266
    Illegal NgoMIV site found at 2643
    Illegal NgoMIV site found at 3397
    Illegal NgoMIV site found at 4717
    Illegal NgoMIV site found at 5000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2608
    Illegal SapI.rc site found at 4566
    Illegal SapI.rc site found at 4776


Design Notes

After meeting with the PI we decided to use the CMV promoter which is a strong promoter. We also expanded the screening selectivity by incorporating a kanamycin/neomycin resistance gene.


Source

the parts created by 2022 iGEM Tokyo Tech, BBa_K4419025.

References