Coding

Part:BBa_K4825048

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


COX6-LEU2

This compostie part contains the part COX6 BBa_K4825013 and LEU2 BBa_K4825010. COX6 encodes cytochrome c oxidase subunit VI in S. cerevisiae, located in the mitochondria. LEU2 encodes 3-isopropylmalate dehydrogenase, which is involved in leucine biosynthesis pathway to catalyze the conversion of beta-isopropylmalate into alpha-ketoisocaproate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 151
    Illegal EcoRI site found at 1090
    Illegal AgeI site found at 703
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 437
    Illegal SapI.rc site found at 421


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Categories
Parameters
None