Coding

Part:BBa_K4825043

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


tHMG1-IDi

This composite part contains truncated hydroxymethylglutaryl-CoA reductase (tHMG1) BBa_K4825000 and Isopentenyl-diphosphate delta-isomerase (IDi) BBa_K4825001 to increase the production of alpha-pinene. The part tHMG1 is responsible for catalyzing the reaction from HMG-CoA to mevalonate in the mevalonate pathway (MVA pathway). And the part IDi is and isomerase that catalyzes the conversion of the relatively unreactive isopentyl pyrophosphate (IPP) to the more reactive dimethylallyl pyrophosphate (DMAPP).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1456
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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