Coding

Part:BBa_K4825029:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


ERG20ww-FL-t48PS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
    Illegal BamHI site found at 2145
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1780


Design Notes

In order to optimize the efficiency of transformation(GPP to a-pinene), a flexible linker GGGGSGGGGS (FL) is introduced to link the C-terminus of ERG20ww and the N-terminus of t48PS. As a result, GPP synthase and pinene synthase would fuses together, thus the allocation of the supply of GPP could be enhanced in the target pathway.


Source

Saccharomyces cerevisiae and Pinus taeda

References

CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188