Part:BBa_K4825029

ERG20ww-FL-t48PS

The part originally contains the part ERG20ww BBa_K4825002 and t48PS BBa_K4825003. ERG20 encodes for farnesyl pyrophosphate synthase(FPPS), which is responsible for converting geranyl pyrophosphate (GPP) to farnesyl pyrophosphate (FPP). In our engineering design, we overexpressed ERG20ww in S. cerevisiae, in which the 96th amino acid, F, and the 127th amino acid, N of the part were both mutated to W amino acid in order to decrease the formation of FPP while maintaining its function to increase the supply of GPP. t48PS encoding for alpha-pinene synthase is a heterologous gene which is originally from Pinus taeda. It is responsible for the conversion from GPP to alpha-pinene. To increase the production, the 48 amino acids at the N-terminus of the part is truncated.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1308
  Illegal BamHI site found at 2145
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1780