Coding

Part:BBa_K4825026

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


XylR-NLS

"This part consists of XylR, the regulatory protein of xylose operon, and the nuclear localization signal (NLS), together regulating the behaviors of the xylose promoters, of which in our project, pXyl.

In the presence of xylose, the transcriptional factor XylR will regulate the transcription controlled by the xylose operon through positive regulation. Meanwhile, the introduction of NLS, a type of signal peptide, allows the karyophilic protein to get into the cell's nuclear without itself being cleaved.

This part inspires the kill switch design of our project by the diversed function of xylose operon that can accomplish a versatile design of gene regulation. "

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


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