Coding
Part:BBa_K4825026
Designed by: Cai Luxi Group: iGEM23_GreatBay-SCIE (2023-10-11)
XylR-NLS
"This part consists of XylR, the regulatory protein of xylose operon, and the nuclear localization signal (NLS), together regulating the behaviors of the xylose promoters, of which in our project, pXyl.
In the presence of xylose, the transcriptional factor XylR will regulate the transcription controlled by the xylose operon through positive regulation. Meanwhile, the introduction of NLS, a type of signal peptide, allows the karyophilic protein to get into the cell's nuclear without itself being cleaved.
This part inspires the kill switch design of our project by the diversed function of xylose operon that can accomplish a versatile design of gene regulation. "
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 235
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |