Coding

Part:BBa_K4825015:Design

Designed by: Ye Siyi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


ARO10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

Saccharomyces cerevisiae

References

"1. Hammer SK, Zhang Y, Avalos JL. Mitochondrial Compartmentalization Confers Specificity to the 2-Ketoacid Recursive Pathway: Increasing Isopentanol Production in Saccharomyces cerevisiae. ACS Synth Biol. 2020 Mar 20;9(3):546-555. doi: 10.1021/acssynbio.9b00420. Epub 2020 Feb 21. PMID: 32049515. 2. Yuan J, Chen X, Mishra P, Ching CB. Metabolically engineered Saccharomyces cerevisiae for enhanced isoamyl alcohol production. Appl Microbiol Biotechnol. 2017 Jan;101(1):465-474. doi: 10.1007/s00253-016-7970-1. Epub 2016 Nov 15. PMID: 27847988."