Coding

Part:BBa_K4825013:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


COX4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to allow the expression of Leu4 in the mitochondria


Source

Saccharomyces cerevisiae

References

"ACS Synth. Biol. 2020, 9, 3, 546–555 Publication Date:February 12, 2020

https://doi.org/10.1021/acssynbio.9b00420"