Coding

Part:BBa_K4825013

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


COX4

The terminal enzyme in the mitochondrial respiratory chain is COX. COX contains 13 mitochondrial and nuclear encoded subunits. The nuclear encoded subunits have yet unknown function but are hypothesized to play a role in constructing and regulating the complex. The mitochondrial encoded subunits aids in the performance of the ETC and proton pumping activities. The N- terminal localization signals from COXIV is used to target Leu4p to mitochondria, and the first 26 amino acids from COX4 appends to the N-terminus of the corresponding open reading frame.


Usage and Biology

The gene encodes the subunit IV isoform 1 which is a nuclear encoded subunit in the human mitochondrial respiratory chain located at the 3’ of the NOC4 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None