Coding

Part:BBa_K4825008:Design

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


JF m


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

P10L mutation is made from the signal peptide JF to improve the production of heterologous proteins in S.cerevisiae as a universal signal peptide.


Source

Kluyveromyces marxianus

References

Zhou J, Zhu P, Hu X, Lu H, Yu Y. Improved secretory expression of lignocellulolytic enzymes in Kluyveromyces marxianus by promoter and signal sequence engineering. Biotechnol Biofuels. 2018 Aug 29;11:235. doi: 10.1186/s13068-018-1232-7. PMID: 30279722; PMCID: PMC6116501.