Part:BBa_K4825003

PS

pinene synthase (PtPS) is a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains N-terminal transport peptides (1-52 loci amino acid residues) in the native host and is responsible for targeting translation products to plastid, however this function is not required in yeast, and the N-terminal transport peptide might cause erring location of the enzyme, decrease in solubility or wrongful folding of the enzyme and affect the efficiency in yeasts, so the N-terminal of PtPS is truncated to form t48PtPS

Usage and Biology

a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains n-terminal transport peptides in the native host and is responsible for targeting translation products to plastid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 223
  Illegal BamHI site found at 1060
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 695