Plasmid
Part:BBa_K4823012:Design
Designed by: Zike Yao Group: iGEM23_HKSSC (2023-10-10)
PA1897_EGFP_mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 789
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1724
Design Notes
we have characterized it to (correcting) PA1897 as promoter. We have knocked out the Lac operon to ensure the EGFP gene is fully regulated.
Source
Escherichia coli BL21