Plasmid

Part:BBa_K4823007

Designed by: Zike Yao   Group: iGEM23_HKSSC   (2023-10-10)


pET_21a (+) _qSCR

The pET-21a (+) is a plasmid backbone. It is used for protein expression and purification in E.coli and other cells. It is good for large quantities of protein expression in E. coli and other cells because it has the powerful T7 promoter. It is ampicillin resistant, so competent cells carrying it can grow on the amp plates successfully. qSCR is a gene that we use for expressing translational factor qSCR. The qSCR molecule binds to AHL molecule, the complex formed will upregulate PA1897 promoter. It is our detection unit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None