Coding

Part:BBa_K4818026:Design

Designed by: Camille Bacquié   Group: iGEM23_INSAENSLyon1   (2023-10-05)


dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain. The 3 fused proteins dcas9-CDA1-UGI are translated into a single protein thanks to the RBS site placed in front of dCas9 (part nb BBa_K4818025).

Source

From the Streptococcus pyogenes

References