Part:BBa_K4818025

RBS

In molecular biology, "RBS" stands for "Ribosome Binding Site." The RBS is a crucial element within messenger RNA (mRNA) that plays a key role in protein synthesis, particularly in prokaryotic organisms like bacteria. It is also sometimes referred to as the "Shine-Dalgarno sequence" in bacterial genetics.

Here's how the RBS works and its significance in protein synthesis:

1. **Location in mRNA**: The RBS is typically located upstream of the start codon (AUG) of a gene in the mRNA molecule. In bacteria, the RBS is often situated a few nucleotides upstream of the AUG start codon.

2. **Ribosome Binding**: The primary function of the RBS is to serve as a recognition site for the ribosome, a cellular organelle responsible for protein synthesis. The ribosome is composed of two subunits: the small subunit and the large subunit. The RBS sequence in the mRNA binds specifically to a complementary sequence in the small ribosomal subunit.

3. **Initiation of Translation**: When the ribosome, with its small subunit bound to the RBS, encounters the mRNA molecule, it helps position the ribosome correctly for the initiation of translation. Translation is the process by which the ribosome reads the mRNA and assembles a corresponding protein by linking together amino acids according to the genetic code.

4. **Start Codon Recognition**: The ribosome, guided by the RBS, moves along the mRNA until it reaches the start codon (usually AUG), which codes for the initiation of protein synthesis. The ribosome then begins the process of translating the mRNA into a protein, adding amino acids one by one in accordance with the genetic code.

In summary, the Ribosome Binding Site (RBS) is a specific sequence in mRNA that facilitates the binding of ribosomes and the initiation of protein synthesis in prokaryotic organisms, especially bacteria. It plays a crucial role in ensuring that the ribosome starts translating the mRNA at the correct location, which is typically the start codon, thus enabling the accurate synthesis of proteins based on the genetic information encoded in the mRNA.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]