Part:BBa_K4817012:Experience

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Applications of BBa_K4817012

Figure.3 The degradation effect of DisH on different biofilms [A:The degradation effect of DisH on DH5α biofilm(24h), B: The degradation effect of DisH on BL21（DE3）-pET28a biofilm(24h), C: The degradation effect of DisH on SRB biofilm(7h)]

DiSH has good degradation effects on the biofilms of E.coli BL21(DE3), E.coli DH5α and SRB

Figure.4 Effect of BL21(DE3)-DisH on biofilm formation

The engineered bacterial biofilms were collected and plated. The number of attached bacteria to the engineered bacterial biofilm containing the plasmid pET-28a-DisH was minimal, indicating that DisH can inhibit biofilm formation.

Figure.5 α-GC Activity Assay results of DisH（left：p-nitrophenol concentration(nmol/mL) assay; right: GC activity）

The enzyme activity of the crude protein extract was measured, and the results showed that the enzyme activity of DisH was low.

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