Coding
NirS

Part:BBa_K4816011:Design

Designed by: Julia Alexander and Matias Brezina   Group: iGEM23_Bonn-Rheinbach   (2023-10-04)


Nitrite Reductase (NirS) from Paracoccus denitrificans PD1222


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 505
    Illegal EcoRI site found at 1446
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 505
    Illegal EcoRI site found at 1446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 505
    Illegal EcoRI site found at 1446
    Illegal BglII site found at 275
    Illegal BglII site found at 1493
    Illegal BamHI site found at 123
    Illegal XhoI site found at 139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 505
    Illegal EcoRI site found at 1446
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 505
    Illegal EcoRI site found at 1446
    Illegal NgoMIV site found at 405
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 877
    Illegal NgoMIV site found at 1585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 835
    Illegal SapI site found at 1425


Design Notes

Only active sites like nirS were needed for the project, not the entire enzyme.


Source

Found on KEGG https://www.genome.jp/entry/pde:Pden_2487

References