Composite

Part:BBa_K4814006:Design

Designed by: CHIEN-YUEH LIU   Group: iGEM23_PuiChing-Macau   (2023-09-28)


ATRIP-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1877
    Illegal BsaI.rc site found at 2065
    Illegal SapI site found at 2889


Design Notes

We have to add flexible linker to fuse the targeted protein and the fluorescent protein.


Source

CMV promoter: BBa_I712004 ATRIP_human: https://www.uniprot.org/uniprotkb/Q8WXE1/entry EGFP: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC146266/

References