Coding

Part:BBa_K4812023:Design

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-12)


LysS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1360
    Illegal PstI site found at 1252
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1360
    Illegal PstI site found at 1252
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1360
    Illegal BglII site found at 393
    Illegal BglII site found at 650
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1360
    Illegal PstI site found at 1252
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1360
    Illegal PstI site found at 1252
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 424


Design Notes

Overexpression of LysS is desired for gene code expansion, hence LysS was chosen instead of LysU.


Source

lysS K12 MG1655

References