Primer

Part:BBa_K4812017

Designed by: Fatima Rebh   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-10)


Site-directed Mutagenesis of Scl2.28 → Scl2.28(GFPGER) Forward

The forward primer is designed to modify, via site-directed mutagenesis, the E. coli-optimized Scl2.28 sequence. The resultant sequence, Scl2.28(GFPGER), contains a Gln → Pro mutation and a Leu → Phe mutation, which introduce GFPGER, an integrin-binding site, to the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 23
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None