Primer

Part:BBa_K4812015

Designed by: Fatima Rebh   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-10)


Site-directed Mutagenesis of Scl2.28 → Scl2.28(GLPGER) Forward

The primer is designed to modify, via site-directed mutagenesis, the E. coli-optimized Scl2.28 sequence. The resultant sequence, Scl2.28(GLPGER), contains a Gln → Pro mutation, which introduces GLPGER, an integrin-binding site to the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None