Part:BBa_K4812012:Design

Scl2.28-1_F4_Forward

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Minimum overlap for Gibson Assembly was set to 25 nt. Minimum primer length was 18 nt.

Source

Primer Designed using NEB Assembly Tool. Contains part of genomic sequence of Scl2.28.