Coding

Part:BBa_K4812001:Experience

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-07)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4812001

Scl2.28 was cloned into pQE80L-Kan vector and used to transform BL21. pQE80L adds a 6His tag on the N-terminus of the protein. Expression was induced using IPTG. The following images after Comaissie stain of the SDS page show the protein present around the expected band of 35 kDa. Further details on our wiki.

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User Reviews

UNIQbef15cd07f74fd28-partinfo-00000000-QINU UNIQbef15cd07f74fd28-partinfo-00000001-QINU