RNA

Part:BBa_K4811024:Design

Designed by: Kasper Krunderup Jakobsen   Group: iGEM23_DTU-Denmark   (2023-10-02)


TMS(PFOA1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Multiple versions of a PFOA-sensitive TMS were attempted, in this instance, the entire aptamer from J. Park et. al. was included into the D-loop.


Source

The TMS was developed by Avishek Paul during their PhD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.

The PFOA-sensitive aptamer was developed via SELEX , see the publication "Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water", Junyoung Park, Kyung-Ae Yang, Yongju Choi, Jong Kwon Choe, Environment International, 2022, 158 107000.

References